H2-D1 Mouse Monoclonal Antibody [Clone ID: 27-11-13S]

CAT#: AM08082PU-N

H2-D1 mouse monoclonal antibody, clone 27-11-13S, Purified

Specifications

Product Data

• Clone Name: 27-11-13S
• Applications: CT, FC
• Recommended Dilution: Flow Cytometry (See Ref.2)
  Immunohistochemistry: Acetone-Fixed, Frozen Tissue Sections only.
  Complement-mediated cytotoxicity (See Ref.1,2)
  Immunocytochemistry (See Ref. 3).
  CMCD (Reported in Ref.1).
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BDF1 mouse splenocytes. (Ref.1)
• Specificity: This antibody recognizes the alpha 3 domain of H-2D^b class I MHC antigen. It cross-reacts with the alpha 3 domain of H-2L^d, H-2D^q and H-2L^q, but not H-2K^d or H-2D^d.
  Reactivity with haplotypes k, f, p, r, and s has not been observed.
  Customer feedback: Clone 27-11-13S does not cross-react with Human cells.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Database Link:
  Entrez Gene 14964 Mouse
• Background: In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen).
  MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant beta 2 Microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
• Synonyms: H-2D(B), H2-D1