Cd86 Rat Monoclonal Antibody [Clone ID: 2D10]

CAT#: AM08084FC-N

Cd86 rat monoclonal antibody, clone 2D10, FITC

Product Images

Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 ug/10e6 cells.

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  BA/2 Mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat anti-Mouse CD86 Antibody

Specifications

Product Data

• Clone Name: 2D10
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells.
  Identification and enumeration of CD86+ cells. (Ref.1,2,4,6,7).
  Immunohistochemistry on Frozen Sections (Ref.4).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse B cell lymphoma cell line 5C2
• Specificity: This antibody recognises CD86, the B7-2 costimulatory molecule and a ligand for CD28 and CD152.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Gene Name: Mus musculus CD86 antigen (Cd86)
• Database Link:
  Entrez Gene 12524 Mouse
• Background: CD86, also known as B72, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease. CD86 interacts with HHV8 (KSHV) MIR2 protein.
• Synonyms: CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1