MHC Class I H-2 Kk Mouse Monoclonal Antibody [Clone ID: 16-3-22S]

CAT#: AM08085PU-N

MHC Class I H-2 Kk mouse monoclonal antibody, clone 16-3-22S, Purified

Specifications

Product Data

• Clone Name: 16-3-22S
• Applications: CT, FC, IHC
• Recommended Dilution: Flow Cytometry. (Ref.1)
  Immunohistochemistry (Acetone-Fixed, Frozen Sections only).
  Complement-mediated Cytotoxicity. (Ref.1)
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H.SW mouse splenocytes.
• Specificity: This antibody reacts with the H-2Kk MHC class I alloantigen.
  Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. (Ref.2) It does not react with other (e.g., b, d, q) haplotypes.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
• Synonyms: H2-K1, H2-K, H-2K(K)