Lamp1 Rat Monoclonal Antibody [Clone ID: 1D4B]

CAT#: AM08087PU-N

Lamp1 rat monoclonal antibody, clone 1D4B, Purified

Specifications

Product Data

• Clone Name: 1D4B
• Applications: EM, FC, IHC, IP, WB
• Recommended Dilution: Flow cytometry: < / = 1 µg/10e6 cells. (Ref.5)
  Immunohistochemistry on Frozen Sections. (Ref.5)
  Immunoaffinity Chromatography. (Ref.2)
  Western blot. (Ref.5)
  Immunoprecipitation. (Ref.1,3)
  Immunoelectron Microscopy. (Ref.6)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Plasma membrane fraction of mouse embryo NIH3T3 cell line. (Ref.1)
• Specificity: This antibody is specific to CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa).
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Database Link:
  Entrez Gene 16783 Mouse
• Background: CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. (Ref.1) It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. (Ref.2)
  CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. (Ref.3)
  LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. (Ref.4)
• Synonyms: LAMP-1, LAMPA, LGP120