CD3E Mouse Monoclonal Antibody [Clone ID: PPT3]

CAT#: AM08104FC-N

CD3E mouse monoclonal antibody, clone PPT3, FITC

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and stained with Mouse anti-Pig CD3e-FITC. Lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: PPT3
• Applications: FC
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells (Ref.1)
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified CD3 molecules from porcine thymus
• Specificity: This antibody recognizes Porcine CD3e (Mr. 23 kDa).
  The Monoclonal antibody PPT3 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Gene Name: -
• Background: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains.
• Synonyms: T3/Leu-4