CD8A Mouse Monoclonal Antibody [Clone ID: 76-2-11]

CAT#: AM08107FC-N

CD8A mouse monoclonal antibody, clone 76-2-11, FITC

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and double stained with Mouse anti-Pig CD8a-FITC and Mouse anti-Pig CD4a-PE. Lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 76-2-11
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1-3)
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody reacts with Porcine CD8 alpha (Mr. 32-34 kDa). (Ref-1-3)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 396627 Pig
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.
• Synonyms: CD8 alpha chain, CD8A, MAL