CD5 Mouse Monoclonal Antibody [Clone ID: f43]

CAT#: AM08111RP-N

CD5 mouse monoclonal antibody, clone f43, PE

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic cat were double-stained with Mouse anti-Feline CD4-FITC (Cat.AM08113FC-N) and Mouse anti-Feline CD5-PE. Small lymphocytes were then gated and analyzed by two-color flow

Specifications

Product Data

• Clone Name: f43
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.1-2)
• Reactivities: Feline
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: Specific to Feline CD5. (Mr. 67kDa)
  This antibody is useful as a pan T Cell Marker. (Ref.1)
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Background: In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
• Synonyms: LEU1, LEU-1, Ly-1, Lyt-1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5