CD8A Mouse Monoclonal Antibody [Clone ID: fCD8]

CAT#: AM08112RP-N

CD8A mouse monoclonal antibody, clone fCD8, PE

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic Cat were double-stained with Mouse anti-Feline CD5-FITC (Cat.AM08111FC-N) and Mouse anti-Feline CD8-PE. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScan (TM) flow cytometry (BDIS, San Jose, CA). Amount Used: 0.2 ug/10e6 cells.

Specifications

Product Data

• Clone Name: fCD8
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.1-2)
• Reactivities: Feline
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Feline CD8 (Mr. 70 kDa) (38/31 kDa under reducing conditions).
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: 0.1 mg/ml
• Conjugation: PE
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL