CD4 Mouse Monoclonal Antibody [Clone ID: 3-4F4]

CAT#: AM08113FC-N

CD4 mouse monoclonal antibody, clone 3-4F4, FITC

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic Cat were double-stained with Mouse anti-Feline CD4-FITC and Mouse anti-Feline CD8-PE. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScan (TM) flow cytometry (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 3-4F4
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1-3)
• Reactivities: Feline
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Feline CD4 (Mr. 65 kDa)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3