T Cell Receptor / TCR gamma/delta Mouse Monoclonal Antibody [Clone ID: TCR1]

CAT#: AM08117PU-N

T Cell Receptor / TCR gamma/delta mouse monoclonal antibody, clone TCR1, Purified

Specifications

Product Data

• Clone Name: TCR1
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry. (Ref.1-3)
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). (Ref.5)
  Immunoprecipitation. (Ref.1-3)
  In ovo depletion of gamma delta T cells. (Ref.6,7)
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes TCR gamma/delta.
  It precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on Chicken peripheral blood T cells. (Ref.1,2)
  Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma/delta T cells. The frequency of TCR gamma/delta cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma/delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma/delta T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.
• Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG, TCR gamma, TCR delta