CD28 Mouse Monoclonal Antibody [Clone ID: AV7]

CAT#: AM08121FC-N

CD28 mouse monoclonal antibody, clone AV7, FITC

Product Images

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD28-FITC and Mouse anti-Chicken CD3-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: AV7
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Gene Name: CD28 molecule
• Database Link:
  Entrez Gene 396249 Chicken
• Background: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)
• Synonyms: TP44