Chicken Lambda light chain Mouse Monoclonal Antibody [Clone ID: L-1]

CAT#: AM08129FC-N

Chicken Lambda light chain mouse monoclonal antibody, clone L-1, FITC

Specifications

Product Data

• Clone Name: L-1
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells.
  Immunofluorescence.
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Lambda light chains of Chicken Immunoglobulin. (Ref.1)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
• Synonyms: Ig lambda chain C regions