MHC Class I Mouse Monoclonal Antibody [Clone ID: F21-2]

CAT#: AM08130FC-N

MHC Class I mouse monoclonal antibody, clone F21-2, FITC

Product Images

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double-stained with Mouse anti-Chicken MHC Class I-FITC and Mouse anti-Chicken IgM-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jos

Specifications

Product Data

• Clone Name: F21-2
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1-4)
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~ 68 kDa). It also reacts with Turkey MHC Class I.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.1-3) Fibroblasts or neurons however only show a low level of antigen.
• Synonyms: MHC Class 1, HLA Class 1