MHC Class I Mouse Monoclonal Antibody [Clone ID: F21-2]

CAT#: AM08130PU-N

MHC Class I mouse monoclonal antibody, clone F21-2, Purified

Specifications

Product Data

• Clone Name: F21-2
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry (Ref.1-4).
  Immunoprecipitation (Ref.1-3).
  Immunohistochemistry on Frozen Sections.
  Western Blot.
  Immunoaffinity Chromatography.
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~ 68 kDa). It also reacts with Turkey MHC Class I.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.1-3)Fibroblasts or neurons however only show a low level of antigen.
• Synonyms: MHC Class 1, HLA Class 1