LOC396098 (Bu1a + Bu1b) Mouse Monoclonal Antibody [Clone ID: AV20]

CAT#: AM08139FC-N

LOC396098 (Bu1a + Bu1b) mouse monoclonal antibody, clone AV20, FITC

Product Images

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD3-PE and Mouse anti-Chicken Bu-1-FITC. Lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDB, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: AV20
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.2)
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens. (Ref.1)
• Specificity: This antibody recognizes a monomorphic determinant on the Bu-1 B cell associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. It also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. (Ref.1-2) No Cross reactivity is observed with Turkey cells when analyzed by Immunofluorescent staining and Flow Cytometry.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Gene Name: B6.1
• Database Link:
  Entrez Gene 396098 Chicken
• Background: The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance.
• Synonyms: B-Cell Marker