CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: DX3]

CAT#: AM08166BT-N

CD95 (FAS) mouse monoclonal antibody, clone DX3, Biotin

Specifications

Product Data

• Clone Name: DX3
• Applications: FC
• Recommended Dilution: Flow Cytometry: 10 µL/10e6 cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human CD95 transfected L cells
• Specificity: This antibody recognizes CD95/Fas/Apo-1.
  Crosslinking of CD95 by the Monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95. (Ref.1-5)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
• Background: CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6