GM CSF (CSF2) Rat Monoclonal Antibody [Clone ID: BVD2-21C11]

CAT#: AM08177BT-N

GM CSF (CSF2) rat monoclonal antibody, clone BVD2-21C11, Biotin

Specifications

Product Data

• Clone Name: BVD2-21C11
• Applications: ELISA, FC, IF
• Recommended Dilution: ELISA Detection: Biotinylated Monoclonal antibody AM08177BT-N is the preferred detection antibody in a Sandwich ELISA for quantifying human GM-CSF protein levels. (Ref.1-4). It should be paired with purified with
  purified recombinant human GM-CSF as the standard.
  Immunofluorescence/Flow Cytometry: This antibody is useful for Intracytoplasmic staining and Flow Cytometric analysis to identify and enumerate GM-CSF-positive cells within mixed cell populations.
  Recombinant human GM-CSF (Cat#AM08177PU-N should be used as a Specificity Control.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: E. coli-expressed human GM-CSF.
• Specificity: This antibody is specific to Human Granulocyte/Macrophage–Colony Stimulating Factor (GM-CSF)1-4. The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a Neutralizing antibody).
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1437 Human
• Background: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
• Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin