CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

CAT#: AM08244AF-N

CD18 (ITGB2) mouse monoclonal antibody, clone MEM-48, Azide Free

Product Images

CD18 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung.

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  CD18 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen.

Specifications

Product Data

• Clone Name: MEM-48
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 2 µg/ml.
  Func.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Immunoprecipitation: 2 µg/ml.
  Western Blot: 2 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia .
• Specificity: Recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kD type I transmembrane protein expressed on all leukocytes.
• Formulation: PBS, pH~7.4 without preservatives
  State: Azide Free
  State: Liquid (0.2 µm sterile filtered) purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens integrin subunit beta 2 (ITGB2), transcript variant 1
• Database Link:
  Entrez Gene 3689 Human
• Background: CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1.
  Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. IIntegrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
• Synonyms: Integrin beta-2, MFI7
• Note: Predicted Molecular Weight: 85 kDa

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis