CD55 / DAF Mouse Monoclonal Antibody [Clone ID: MEM-118]

CAT#: AM08312PU-N

CD55 / DAF mouse monoclonal antibody, clone MEM-118, Purified

Product Images

Skin, pilosebaceous unit: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: MEM-118
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Immunoprecipitation.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: HPB-ALL Human T cell line.
• Specificity: Recongizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.
• Formulation: PBS ~ pH 7.4 containing 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 1 mg/ml
• Purification: Size Exclusion Chromatography.
• Background: Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins.
  This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.