Myog (30-224) Mouse Monoclonal Antibody [Clone ID: 5FD]

CAT#: AM08353PU-N

Myog (30-224) mouse monoclonal antibody, clone 5FD, Aff - Purified

Product Images

at 10 ug/ml staining Myogenin in Skeletal muscle by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue.

Specifications

Product Data

• Clone Name: 5FD
• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 5 µg/ml.
  Immunohistochemistry on Frozen Sections: 5 - 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 2 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A recombinant GST fusion protein corresponding to amino acids 30-224 of Rat Myogenin was used as immunogen.
• Specificity: This antibody recognizes Myogenin at aa 30-224.
• Formulation: PBS containing 0.05% Sodium Azide as preservative and 0.05% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Protein G Chromatography.
• Database Link:
  Entrez Gene 29148 Rat
• Background: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of ~34 kDa by SDS-PAGE.
• Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3