Caspase 3 (CASP3) (Full length) Mouse Monoclonal Antibody [Clone ID: 31A1067]

CAT#: AM08377PU-N

Caspase 3 (CASP3) (Full length) mouse monoclonal antibody, clone 31A1067, Purified

Product Images

Western blot analysis of Caspase-3 in HeLa cells. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points.

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  Western blot analysis of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in Human, Mouse and Rat heart lysate, respectively.

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  Caspase-3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 31A1067
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 2 - 3 µg/ml.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Full-length recombinant Human Caspase-3
• Specificity: The antibody recognizes an epitope in the large domain subunit of Caspase-3.
  As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
• Formulation: PBS containing 0.05% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 836 Human
• Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase 6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa.
• Synonyms: CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1