Insulin (INS) Mouse Monoclonal Antibody [Clone ID: 8E2]

CAT#: AM08397HR-N

Insulin (INS) mouse monoclonal antibody, clone 8E2, HRP

Product Images

AM08397HR-N Insulin Antibody stainig of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 8E2
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human Insulin
• Specificity: Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30%) and porcine insulin. Does not cross-react with free C-peptide.
• Formulation: PBS, pH 7.4 containing 0.05% Proclin300
  Label: HRP
  State: Liquid purified Ig fraction
  Label: Horseradish Peroxidase
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3630 Human
• Background: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
• Synonyms: INS