MHV NSP9 (Strain A59) Mouse Monoclonal Antibody [Clone ID: 2C6.H1]

CAT#: AM08450PU-N

MHV NSP9 (Strain A59) mouse monoclonal antibody, clone 2C6.H1, Purified

Product Images

Western blotting using anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no crossreactivity with SARS-CoV-infected Vero cells. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown

• 

  Immunofluorescence microscopy using anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% paraformaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 (610-111-121) was used. Personal Communication, Eric Snijder

Specifications

Product Data

• Clone Name: 2C6.H1
• Applications: IF, WB
• Recommended Dilution: Western blot: 1:1,000 (Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 in the appropriate cell lysate or extract).
  Immunofluorescence: 1:1,000 (Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min).
  
  
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: E. coli derived fulllength MHV-A59 nsp9 protein.
• Specificity: This antibody is directed against the MHV-A59 nsp9 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: 2.1 mg/ml (by UV absorbance at 280 nm)
• Purification: Protein A chromatography
• Background: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
• Synonyms: Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus