CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: 3G4]

CAT#: AM09001PU-N

CD45 (PTPRC) mouse monoclonal antibody, clone 3G4, Purified

Product Images

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: CD45 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thymus at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: CD45 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 3G4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/3,000), recommended starting dilution is 1/2000.
  Immunohistochemistry on Paraffin Sections (10 µg/ml). Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human CD45 (1029-1249 aa) purified from E. coli.
• Specificity: The antibody recognizes Human CD45.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180,000 to 220,000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200