CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: 3G4]

CAT#: AM09001PU-N

CD45 (PTPRC) mouse monoclonal antibody, clone 3G4, Purified


USD 340.00

2 Weeks*

Size
    • 100 ul

Product Images

Specifications

Product Data
Clone Name 3G4
Applications ELISA, IHC, WB
Recommended Dilution ELISA.
Western blot (1/1,000-1/3,000), recommended starting dilution is 1/2000.
Immunohistochemistry on Paraffin Sections (10 µg/ml). Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant human CD45 (1029-1249 aa) purified from E. coli.
Specificity The antibody recognizes Human CD45.
Other species not tested.
Formulation PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State: Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Protein-G affinity chromatography
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180,000 to 220,000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.