Eph receptor A2 (EPHA2) Mouse Monoclonal Antibody [Clone ID: 3D7]

CAT#: AM09008PU-S

Eph receptor A2 (EPHA2) mouse monoclonal antibody, clone 3D7, Purified

Product Images

Western blot analysis on mouse brain: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Testis stained with EPHA2 antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Thyroid stained with EPHA2 antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 antibody (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 3D7
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000).
  Immunohistochemistry (1/100-1/200).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human EphA2 (559-976 aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse EPHA2.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens EPH receptor A2 (EPHA2)
• Database Link:
  Entrez Gene 13836 MouseEntrez Gene 1969 Human
• Background: The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTK). Eph RTKs and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
• Synonyms: Ephrin type-A receptor 2, Epithelial cell kinase, Eph receptor A2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance