NANOG (1-154) Mouse Monoclonal Antibody [Clone ID: 5A10]

CAT#: AM09023PU-N

NANOG (1-154) mouse monoclonal antibody, clone 5A10, Purified

Product Images

Western blot analysis: The cell lysates of NIH3T3 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Paraffin Embedded Sections of Human seminoma tissue were incubated with anti-Human Nanog (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 5A10
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000).
  Flow Cytometry.
  Immunohistochemistry on Paraffin Sections (1/50-1/100).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human Nanog (1-154 aa) purified from E. coli
• Specificity: The antibody recognizes NANOG.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-G affinity chromatography
• Gene Name: Nanog homeobox
• Database Link:
  Entrez Gene 79923 Human
• Background: Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
• Synonyms: FLJ12581; FLJ40451; hNanog