TDP43 (TARDBP) Mouse Monoclonal Antibody [Clone ID: k1B8]

CAT#: AM09038PU-S

TDP43 (TARDBP) mouse monoclonal antibody, clone k1B8, Purified

Product Images

Western blot analysis: Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1/1,000). Proteins were visualized using HRP conjugated Goat anti-Mouse secondary antibody and an ECL detection system.

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  Immunohistochemistry: Paraffin Embedded Sections of Human prostate cancer were incubated with anti-Human TARDBP antibody (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

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  Immunohistochemistry: Paraffin Embedded Sections of Human seminoma tissue were incubated with anti-Human TARDBP antibody (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: k1B8
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Flow Cytometry.
  Immunohistochemistry on Paraffin Sections (1/50-1/100).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human TARDBP (1-260 aa) purified from E. coli.
• Specificity: The antibody recognizes Human TARDBP.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-G Affinity Chromatography
• Gene Name: Homo sapiens TAR DNA binding protein (TARDBP)
• Database Link:
  Entrez Gene 23435 Human
• Background: TAR DNA binding protein (TARDBP), also known as TDP-43, is a cellular protein. TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS). TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.
• Synonyms: TDP43, TDP-43

Reference Data

• Protein Families: Transcription Factors