YWHAB

CAT#: AM09043PU-N

Mouse Monoclonal Anti-YWHAB Antibody, Purified

Product Images

Western blot analysis: Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 beta antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: 14-3-3 beta antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thyroid at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: J2E9
• Applications: IHC, WB
• Recommended Dilution: ELISA, WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human 14-3-3 beta (1-246 aa) purified from E. coli
• Specificity: The antibody recognizes human 14-3-3 beta.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  Shelf life: one year from despatch.
• Predicted Protein Size: 27.9 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• Database Link:
  NP_647539
  Entrez Gene 7529 Human
• Background: The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, ?, ¦Å, ¦Ò, ¦Æ, ¦Ó and ¦Ç that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery.
• Synonyms: GW128; HS1; KCIP-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis