BID (1-195) Mouse Monoclonal Antibody [Clone ID: 4D3]

CAT#: AM09052PU-N

BID (1-195) mouse monoclonal antibody, clone 4D3, Purified

Product Images

The lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BID (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

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  Western blot analysis: The lysates of Jurkat (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BID (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system.

Specifications

Product Data

• Clone Name: 4D3
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Flow Cytometry.
  Immunofluorescence/Immunocytochemistry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human BID (aa 1-195) purified from E. coli
• Specificity: The antibody recognizes Human BID.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2
• Database Link:
  Entrez Gene 637 Human
• Background: BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into the outer mitochondrial membrane. Bax is believed to induce the opening of the mitochondrial voltage-dependent anion channel. This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria leading to activation of caspases.
• Synonyms: p22 BID

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis