HMG1 (HMGB1) Mouse Monoclonal Antibody [Clone ID: J2E1]

CAT#: AM09063PU-S

HMG1 (HMGB1) mouse monoclonal antibody, clone J2E1, Purified

Product Images

Western blot analysis: Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HMGB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate bufferand detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: J2E1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:2,000), recommended starting dilution is 1:1,000.
  Immunohistochemistry (1:100 - 1:300), recommended starting dilution is 1:200.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells.
• Specificity: The antibody recognizes human HMGB1.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens high mobility group box 1 (HMGB1), transcript variant 2
• Database Link:
  Entrez Gene 3146 Human
• Background: High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.
• Synonyms: High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1, Amphoterin

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair