Adipose Triglyceride Lipase (PNPLA2) Mouse Monoclonal Antibody [Clone ID: AT2G2]

CAT#: AM09064PU-S

Adipose Triglyceride Lipase (PNPLA2) mouse monoclonal antibody, clone AT2G2, Purified

Product Images

Western blot analysis: The extracts of mouse kidney (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ATGL (1/1000) (. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL. (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection

Specifications

Product Data

• Clone Name: AT2G2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1000-1/2000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human ATGL (30-504 aa) purified from E. coli.
• Specificity: The antibody recognizes ATGL (PNPLA2).
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Gene Name: Homo sapiens patatin like phospholipase domain containing 2 (PNPLA2)
• Database Link:
  Entrez Gene 66853 MouseEntrez Gene 57104 Human
• Background: Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.
• Synonyms: FP17548, Desnutrin, TTS2.2, IPLA2-zeta

Reference Data

• Protein Families: Transmembrane