RBP4 Mouse Monoclonal Antibody [Clone ID: AT2B4]

CAT#: AM09065PU-S

RBP4 mouse monoclonal antibody, clone AT2B4, Purified

Product Images

Western blot analysis: Cell lysates of HepG2 (60 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RBP4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human RBP4 (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Specifications

Product Data

• Clone Name: AT2B4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:1,000).
  Immunohistochemistry on paraffin-embedded tissues (1:25-1:50).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human RBP4 (19-201 aa) purified from E. coli
• Specificity: The antibody recognizes human RBP4.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens retinol binding protein 4 (RBP4), transcript variant 1
• Database Link:
  Entrez Gene 5950 Human
• Background: Retinol binding protein 4(RBP4) belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.
• Synonyms: RBP-4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein