FKBP52 (FKBP4) Mouse Monoclonal Antibody [Clone ID: AT4D3]

CAT#: AM09071PU-N

FKBP52 (FKBP4) mouse monoclonal antibody, clone AT4D3, Purified

Product Images

Western blot analysis: Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FKBP4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection.

Specifications

Product Data

• Clone Name: AT4D3
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Cell lysates of HeLa (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FkBP4 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  Immunohistochemistry on Paraffin Embedded Tissue (1/50-1/100).
  Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-Human FKBP4 (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human FKBP4 (1-459 aa) purified from E. coli
• Specificity: The antibody recognizes Human FKBP4. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens FK506 binding protein 4 (FKBP4)
• Database Link:
  Entrez Gene 2288 Human
• Background: FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
• Synonyms: FK506-binding protein 4, FKBP52 protein, FKBP59, p59 protein

Reference Data

• Protein Families: Druggable Genome