Casein Kinase 1 alpha (CSNK1A1) Mouse Monoclonal Antibody [Clone ID: AT2E2]

CAT#: AM09078PU-N

Casein Kinase 1 alpha (CSNK1A1) mouse monoclonal antibody, clone AT2E2, Purified

Product Images

Western blot analysis: Cell lysates of HeLa and MCF7 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2E2
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1/1000-1/2000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human Casein Kinase 1 alpha (1-337 aa) purified from E. coli
• Specificity: The antibody recognizes Human Casein Kinase 1 alpha. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens casein kinase 1 alpha 1 (CSNK1A1), transcript variant 2
• Database Link:
  Entrez Gene 1452 Human
• Background: Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex.
• Synonyms: Casein kinase I alpha, CKI-alpha, CK1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway, Wnt signaling pathway