Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: AT1H23]

CAT#: AM09083PU-N

Ribonuclease Inhibitor (RNH1) mouse monoclonal antibody, clone AT1H23, Purified

Product Images

The extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis : The extracts of mouse liver (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: RNH1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: RNH1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: AT1H23
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Immunohistochemistry on Paraffin Sections (5 µg/ml). This RNH1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Ribonuclease inhibitor 1 (RNH1) (7-461 aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse RNH1 (7-461).
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1
• Database Link:
  Entrez Gene 107702 MouseEntrez Gene 6050 Human
• Background: Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.
• Synonyms: Ribonuclease/angiogenin inhibitor 1, RAI, RNH, PRI