TDP1 Mouse Monoclonal Antibody [Clone ID: AT1F2]

CAT#: AM09086PU-S

TDP1 mouse monoclonal antibody, clone AT1F2, Purified

Product Images

Western blot analysis: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human TDP1 antibody (1/1000). Proteins were visualized using a goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.

• 

  Immunohistochemistry: -N TDP1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Small Intestine followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Immunohistochemistry: -N TDP1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: AT1F2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1,000-1/2,000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human TDP1 (1-298 aa) purified from E. coli
• Specificity: The antibody recognizes Human TDP1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Gene Name: Homo sapiens tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1
• Database Link:
  Entrez Gene 55775 Human
• Background: Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.
• Synonyms: Tyrosyl-DNA phosphodiesterase 1

Reference Data

• Protein Families: Druggable Genome