PDK1 (29-436) Mouse Monoclonal Antibody [Clone ID: AT2D6]

CAT#: AM09090PU-N

PDK1 (29-436) mouse monoclonal antibody, clone AT2D6, Purified

Product Images

The extracts of rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: The extracts of rat heart (60 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2D6
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:250 - 1:1,000).
  Immunocytochemistry / Immunoflourescence.
  Flow cytometry.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human PDK1 (29-436 aa) purified from E. coli
• Specificity: The antibody recognizes PDK1 at aa 29-436.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens pyruvate dehydrogenase kinase 1 (PDK1), transcript variant 2
• Database Link:
  Entrez Gene 116551 RatEntrez Gene 5163 Human
• Background: PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.
• Synonyms: Pyruvate dehydrogenase kinase isoform 1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Fc epsilon RI signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway