p21WAF1 (Tumor Suppressor Protein) Mouse Monoclonal Antibody [Clone ID: WA-1]

CAT#: AM09100PU-S

p21 (CDKN1A) mouse monoclonal antibody, clone WA-1, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody (Clone WA-1).

Specifications

Product Data

• Clone Name: WA-1
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/10⁶ cells in 100 µl.
  Immunofluorescence: 1-2 μg/ml.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: HeLa Cells. Skin, colon, or breast carcinoma.
• Reactivities: Chimpanzee, Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant p21 protein
• Specificity: Cellular Localization: Cytoplasm, Nucleus
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21kDa
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
• Synonyms: Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)