alpha smooth muscle Actin (ACTA2) (N-term) Mouse Monoclonal Antibody [Clone ID: ASM-1]

CAT#: AM09118PU-N

alpha smooth muscle Actin (ACTA2) (N-term) mouse monoclonal antibody, clone ASM-1, Purified

Specifications

Product Data

• Clone Name: ASM-1
• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence Microscopy
  Immunoblotting (Western).
  Cytological Material.
  Immunohistochemistry on Frozen and Paraffin-Embedded Tissue.
  Pretreatment: With paraffin embedded sections, protease pretreatment is required prior to antibody application.
  Working Dilution: Ready-to-use for Immunohistochemical application.
  Incubation Time: 1 h at RT; extended with paraffin.
• Reactivities: Bovine, Chicken, Equine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of Actin.
• Specificity: ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
  Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000).
  Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
  Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Purification: Affinity Chromatography on Protein A
• Storage: Store the antibody undiluited at 2-8°C
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens actin, alpha 1, skeletal muscle (ACTA1)
• Database Link:
  Entrez Gene 11459 MouseEntrez Gene 29437 RatEntrez Gene 59 Human
• Background: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
• Synonyms: ACTSA, ACTVS, Alpha-actin-2

Reference Data

• Protein Families: Stem cell - Pluripotency