pan Cytokeratin Mouse Monoclonal Antibody [Clone ID: lu-5]

CAT#: AM09120PU-N

pan Cytokeratin mouse monoclonal antibody, clone lu-5, Purified

Specifications

Product Data

• Clone Name: lu-5
• Applications: IF, IHC
• Recommended Dilution: Immunohistochemistry on frozen and paraffin sections: 1/20.
• Reactivities: Bovine, Chicken, Human, Rat, Xenopus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cells of lung cancer cell line A549 and A2182.
• Specificity: This antibody reacts to Cytoketatin pan.
• Formulation: 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4
  State: Purified
  State: Lyophilised purified Ig
• Reconstitution Method: Restore in 1 ml dist. water
• Purification: Ion exchange chromatography
• Background: Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.
  There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
• Synonyms: pan Keratin, Cytokeratin pan-reactive