Plakophilin 3 (PKP3) Mouse Monoclonal Antibody [Clone ID: PKP3-270.6.2]

CAT#: AM09123SU-N

Plakophilin 3 (PKP3) mouse monoclonal antibody, clone PKP3-270.6.2, Supernatant

Specifications

Product Data

• Clone Name: PKP3-270.6.2
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/500 ECL method.
  Immunofluorescence.
  Immunohistochemistry on frozen sections: Ready-to-use.
• Reactivities: Bovine, Human, Mouse, Xenopus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein (E. coli) of human plakophilin 3
• Specificity: This antibody reacts to Plakophilin 3.
  Positive tissues and culture cells:
  - stratified squamous epithelia: palmar, epidermis, scalp skin
  - stratified epithelia: tongue, vagina, esophagus
  - transitional epithelia: bladder
  - non-stratified epithelia: colon, duodenum, pancreas,
  several glands
  - non-epithelial: reticulum cells of lymph node follicle
  - HaCaT, MCF7, HeLa, HT-29, CaCo 2, Caski, BMGE, MDCK and other
  Negative tissues and cell lines:
  - liver, heart, skeletal muscle, endothelia (vessels)
  - PLC, RD, SV80, HL60, Raji, Jok1, Glioma, K562, 3T3L1, A6, PtK2
• Formulation: State: Supernatant
  State: Liquid culture supernatant
• Gene Name: Homo sapiens plakophilin 3 (PKP3), transcript variant 1
• Database Link:
  Entrez Gene 56460 MouseEntrez Gene 11187 Human
• Background: Plakophilin 3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
• Synonyms: Plakophilin-3