GAPDH Mouse Monoclonal Antibody [Clone ID: H8]

CAT#: AM09140PU-N

GAPDH mouse monoclonal antibody, clone H8, Purified

Product Images

Western blot using GAPDH Antibody Clone H8

Specifications

Product Data

• Clone Name: H8
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Dot blot.
  Western blott: 1/1,000-1/10,000 using ECL Substrate.
• Reactivities: Bacteria, Human, Insect, Mouse, Rabbit, Rat, Yeast
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant GAPDH
• Specificity: Recognizes native and recombinant GAPDH in Human, Mouse, Rat, Rabbit, sf9 Insect cells, Yeast and BL-21 Bacteria.
• Formulation: 0.01M PBS, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.05% Sodium Azide
• Reconstitution Method: Restore in double distilled or deioned H₂O to a concentration of 1.0 mg/ml.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1
• Database Link:
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
• Synonyms: GAPD, CDABP0047

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways