HA Epitope Tag (YPYDVPDYA) Mouse Monoclonal Antibody [Clone ID: HA.C5]

CAT#: AM09142PU-N

HA Epitope Tag (YPYDVPDYA) mouse monoclonal antibody, clone HA.C5, Purified

Product Images

Western blot using HA tag antibody: Western blot analysis of 293 cells transfected with HA Tagged vector (Lane 2) and untransfected control (Lane 1).

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  Immunocytochemistry/Immuno-fluorescence using HA tag antibody: Immunofluorescence staining of HA Tag fusion protein (transcription factor) in a stable expressing cell line (B) and control cell line (A).

Specifications

Product Data

• Clone Name: HA.C5
• Applications: IF, IP, WB
• Recommended Dilution: Western blot: 1/1,000-1/4,000.
  Immunoprecipitation: 1/200.
  Immunostaining: 1/200-1/400.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: A synthetic peptide (YPYDVPDYA) from Influenza Hemagglutinin epitope coupled to KLH.
• Specificity: This monoclonal HA Tag antibody binds to HA-tagged recombinant protein in transfected mammalian cells.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.