EGF Mouse Monoclonal Antibody [Clone ID: S-146]

CAT#: AM09149BT-N

EGF mouse monoclonal antibody, clone S-146, Biotin

Specifications

Product Data

• Clone Name: S-146
• Applications: ELISA
• Recommended Dilution: ELISA: This Biotin-conjugated monoclonal antibody can be used as a Tracer/Detection antibody in a Sandwich ELISA for Human EGF detection in combination with a Capture antibody (Cat.-No DM1012).
  Suggested Capture Coating Dose: 0.3 μg/well (Substrate: TMB).
  If the above suggested conditions are followed approximately 20 pg/ml of EGF in serum/plasma or 15 pg/mL of EGF in cell medium/urine can be detected with an assay range of 0-1000 pg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant human EGF, 6kDa, 53 amino acid residues
• Specificity: This monoclonal antibody reacts with natural and recombinant Human EGF.
  No cross-reactivity with other Human cytokines such as IL-1β, TGF-β1, IL-8, MCP-1, and SAA
• Formulation: 0.01M PBS, pH 7.0 ± 0.1
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% Gelatin
  Preservative: 0.09% Sodium Azide
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Storage:

  Store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens epidermal growth factor (EGF), transcript variant 1
• Database Link:
  Entrez Gene 1950 Human
  UniProt ID P01133
• Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
  Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
• Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton