Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: 315]

CAT#: AM09167PU-N

Interferon gamma (IFNG) mouse monoclonal antibody, clone 315, Aff - Purified

Specifications

Product Data

• Clone Name: 315
• Applications: ELISA
• Recommended Dilution: ELISA: This IFN gamma antibody can be used as a Capture Antibody in Sandwich ELISA applications for Human IFN-gamma Detection in combination with a monoclonal Tracer/Detection antibody (HRP-conjugated, Cat#AM09176HR-N).
  Suggested Capture Coating Dose: 0.3 µg/well.
  Substrate: TMB. If the above suggested conditions are followed approximately 13.1 pg/mL of IFN-gamma in serum/plasma or 7.5 pg/ml of IFN-gamma in cell medium/urine can be detected with an assay range of 0-1600 pg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human Interferon gamma (IFN gamma).
• Specificity: This antibody reacts with natural and recombinant Human IFN-gamma.
• Formulation: 0.01M PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/ml
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interferon, gamma (IFNG)
• Database Link:
  Entrez Gene 3458 Human
  UniProt ID P01579
• Background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
• Synonyms: IFN-gamma, gamma IFN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus