HCV NS3 Mouse Monoclonal Antibody [Clone ID: 806]

CAT#: AM09205PU-N

HCV NS3 mouse monoclonal antibody, clone 806, Aff - Purified

Specifications

Product Data

• Clone Name: 806
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 806) at 0.1-1 µg/ml will allow visualization of 100 ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 Protein.
  No reaction can be seen with Synthetic NS-3. Testing is under reducing and non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Recombinant Chimeric HCV Polyprotein (555 a.a.)
• Specificity: HCV NS-3 antibody (clone 806) reacts with Recombinant NS-3 (aa 1252-1477) and Recombinant Chimeric HCV Polyprotein (60 kDa).
  Cross-Reactivity: No cross reaction can be seen with Recombinant Capsid Protein C (CPC)+Envelope Protein M (EPM) (core) (aa 1-142), Synthetic CPC (aa 1-61), Synthetic NS-3 (aa 1378-1458) and Synthetic NS-4a Protein (aa 1689-1735).
• Formulation: 0.01M PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication.
  The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase.
  The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
• Synonyms: Hepatitis C Virus Serine proteiase NS3