MUC16 Mouse Monoclonal Antibody [Clone ID: 23]

CAT#: AM09242PU-N

MUC16 mouse monoclonal antibody, clone 23, Aff - Purified

Product Images

Staining of methanol-fixed Hela cells by Immuno-fluorescence: Anti-CA 125 mAb clone 23 used at 10μg/ml.

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  Hela cells stained with 10μg/ml anti-CA 125 mAb clone 23 and fluorescence-labelled secondary antibody. Black line represents the histogram of Hela cells stained with control antibody, mouse anti-TB 38Kd antigen mAb clone B12F8.

Specifications

Product Data

• Clone Name: 23
• Applications: ELISA, FC, IF
• Recommended Dilution: ELISA: 0.5 ng/ml or higher can recognize ELISA plates wells coated with CA125 at 5 international units per well.
  Flow Cytometry: 10 μg/ml.
  Immunofluorescence: 10 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human CA125.
• Specificity: Reactive with Human CA125.
• Formulation: 0.01M PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mucin 16, cell surface associated (MUC16)
• Database Link:
  Entrez Gene 94025 Human
  UniProt ID Q8WXI7
• Background: Mucin16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller subunits being described by some investigators.
• Synonyms: MUC16, MUC-16, CA-125, CA 125