Prostate Specific Antigen (KLK3) (free) Mouse Monoclonal Antibody [Clone ID: 108]

CAT#: AM09251PU-N

Prostate Specific Antigen (KLK3) (free) mouse monoclonal antibody, clone 108, Aff - Purified

Specifications

Product Data

• Clone Name: 108
• Applications: ELISA
• Recommended Dilution: ELISA: This monoclonal PSA antibody can be used as Capture antibody in a Sandwich ELISA for Human free PSA detection in combination with a HRP-conjugated Tracer antibody Cat.-No AM09250HR-N that is derived from PSA mAb clone 103 (Cat.-No AM09250PU-N).
  Suggested Capture Coating Dose: 0.5 µg/well.
  Substrate: TMB.
  If the above suggested conditions are followed approximately 22 pg/mL of Free PSA can be detected.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Human Free PSA.
• Specificity: This monoclonal antibody reacts with Human free PSA, but does not react with complexed PSA.
• Formulation: 0.01M PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with Double distilled water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens kallikrein related peptidase 3 (KLK3), transcript variant 3
• Database Link:
  Entrez Gene 354 Human
  UniProt ID P07288
• Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.
• Synonyms: Prostate-specific antigen, Semenogelase, Gamma-seminoprotein, P-30 antigen

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Pathways in cancer, Prostate cancer