ELK1 pSer383 Mouse Monoclonal Antibody [Clone ID: B4A12]

CAT#: AM09315PU-N

ELK1 pSer383 mouse monoclonal antibody, clone B4A12, Aff - Purified

Specifications

Product Data

• Clone Name: B4A12
• Applications: ELISA
• Recommended Dilution: Indirect ELISA: The antibody is reactive to Serine383 phosphorylated Human ELK-1 peptide conjugated with KLH or the peptide- BSA conjugate.
  The suggested antigen Coating quantity is 1 µg/ml of peptide/or peptide conjugating in pH 9.5 carbonate buffer, 100 µl/well.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide of a phosphorylated fragment of ELK1 (pSer383) conjugated with KLH.
• Specificity: Reactive to Serine383 phosphorylated Human ELK-1 peptide fragment conjugated with KLH or the peptide-BSA conjugate.
  Not reactive to unconjugated Serine383 phosphorylated Human ELK-1 peptide, KLH or BSA.
• Formulation: 0.01M PBS, pH 7.0 without preservatives
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ELK1, member of ETS oncogene family (ELK1), transcript variant 1
• Database Link:
  Entrez Gene 2002 Human
  UniProt ID P19419
• Background: ELK1 belongs to the EST transcription factor family. Proteins in the family have a unique DNA binding domain that binds to conserved DNA sequences. Phosphorylation of ELK1 at Serine 383 and 389 by mitogen-activated protein kinases (MAPKs) activates ELK1. The binding of ELK1 to target sequences is also regulated by its co-factor, serum response factor (SRF), by alternative splicing, and by SUMOylation. ELK1 regulates the transcription of c-fos and genes encoding cytoskeleton proteins, and was found to play a role in longterm memory formation, drug addiction and other neurological disorders.
• Synonyms: Elk-1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endometrial cancer, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Prion diseases