RAF1 (N-term) Mouse Monoclonal Antibody [Clone ID: 163]

CAT#: AM09323PU-N

RAF1 (N-term) mouse monoclonal antibody, clone 163, Purified

Specifications

Product Data

• Clone Name: 163
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot: When used at 1μg/mL, will allow visualization of 0.2 μg/lane of recombinant Human Raf-1 (75 kDa).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant Raf-1 (mw:75 kDa)
• Specificity: This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.). Not suitable for the detection of Raf-1 expression and phosphorylation in Hela cells.
• Formulation: 0.01M PBS, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1)
• Database Link:
  Entrez Gene 5894 Human
  UniProt ID P04049
• Background: The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
• Synonyms: C-RAF, Raf-1, cRaf

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway